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Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

Frontiers of Medicine 2022, Volume 16, Issue 4,   Pages 627-636 doi: 10.1007/s11684-020-0815-4

Abstract: Then, based on RNA-seq data from The Cancer Genome Atlas AML cohort, we developed a 300-gene signatureThis study revealed the distinct gene expression pattern of RUNX1 mutations and the underlying

Keywords： RUNX1     gene mutation     acute myeloid leukemia     transcriptional repression     DNA methylation    

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Mutation profiling of 16 candidate genes in

Yang Zhang, Fang Wang, Xue Chen, Wenjing Liu, Jiancheng Fang, Mingyu Wang, Wen Teng, Panxiang Cao, Hongxing Liu

Frontiers of Medicine 2019, Volume 13, Issue 2,   Pages 229-237 doi: 10.1007/s11684-018-0616-1

Abstract: This retrospective analysis aimed to investigate the mutation profile of 16 common mutated genes inWe identified at least 1 mutation in 199 of the 259 samples (76.8%), and 2 or more mutations in 31.7%was the most common mutated gene (16.2%, 42/259), followed by (15.1%, 39/259), (14.7%, 38/259), andDistinct patterns of co-occurrence were observed for different hotspot mutations within the gene:Defining the mutation spectrum and mutation pattern of AML will contribute to the comprehensive assessment

Keywords： leukemia     myeloid     acute     gene     mutation    

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662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)

QU Yanchun, YANG Ze, SUN Liang, JI Linong

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 283-285 doi: 10.1007/s11684-008-0053-7

Abstract: The aim of this paper is to report a new coding variance of the gene, a candidate for autoimmune diseasesWe found the variation in two families with type 2 diabetes mellitus by D-HPLC mutation screening method

Keywords： D-HPLC mutation     development     autoimmune     PCR-RFLP     candidate    

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Analysis of the genomic landscape of primary central nervous system lymphoma using whole-genome sequencing in Chinese patients

Frontiers of Medicine   Pages 889-906 doi: 10.1007/s11684-023-0994-x

Abstract: CD79B mutation was significantly associated with lower PFS, TMSB4X mutation and high expression

Keywords： primary central nervous system lymphoma     whole-genome sequencing     TMSB4X     copy number variation     genemutation    

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Novel mutation c.1210-3C>G in with a poly-T tract of 5T affects mRNA splicing in a Chinese patient

Frontiers of Medicine 2022, Volume 16, Issue 1,   Pages 150-155 doi: 10.1007/s11684-021-0846-5

Abstract: Cystic fibrosis (CF) is a rare autosomal recessive disease with only one pathogenic gene cystic fibrosisOverall, c.1210-3C>G, the newly identified pathogenic mutation in our patient, in combination with T5sequence in cis, affects the CFTR gene splicing and produces nearly no normal transcriptMoreover, this patient carries a p.Gly970Asp mutation, thus confirming the high-frequency of this mutation

Keywords： cystic fibrosis     CFTR     splicing mutation     minigene    

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Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases

Xia Jiahui

Strategic Study of CAE 2000, Volume 2, Issue 11,   Pages 1-11

Abstract:

In this paper, the study on family collection, gene mapping, gene identification and gene functiont (1; 3) (q44; p11) associated with nasopharyngeal cancer was found in 1975 at first, and human TDF geneIn 1998, GJB3, a human genetic neurological deafness gene, was identified using a novel strategy of “Gene Family-Candidate Disease Gene Cloning”，and the paper was published in Nature Geneticswas also cloned from gene function study.

Keywords： genetic disease     gene mapping and cloning     gene familiy-candidate disease gene cloning     genome wide scan     gene function study    

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Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 663-675 doi: 10.1007/s11705-017-1623-5

Abstract: Small interfering RNA (siRNA) therapeutics hold great promise to treat a variety of diseases, as long as they can be delivered safely and effectively into cells. Dendrimers are appealing vectors for siRNA delivery by virtue of their well-defined molecular architecture and multivalent cooperativity. However, the clinical translation of RNA therapeutics mediated by dendrimer delivery is hampered by the lack of dendrimers that are of high quality to meet good manufacturing practice standard. In this context, we have developed small amphiphilic dendrimers that self-assemble into supramolecular structures, which mimic high-generation dendrimers synthesized with covalent construction, yet are easy to produce in large amount and superior quality. Indeed, the concept of supramolecular dendrimers has proved to be very promising, and has opened up a new avenue for dendrimer-mediated siRNA delivery. A series of self-assembling supramolecular dendrimers have consequently been established, some of them out-performing the currently available nonviral vectors in delivering siRNA to various cell types and , including human primary cells and stem cells. This short review presents a brief introduction to RNAi therapeutics, the obstacles to their delivery and the advantages of dendrimer delivery vectors as well as our bio-inspired structurally flexible dendrimers for siRNA delivery. We then highlight our efforts in creating self-assembling amphiphilic dendrimers to construct supramolecular dendrimer nanosystems for effective siRNA delivery as well as the related structural alterations to enhance delivery efficiency. The advent of self-assembling supramolecular dendrimer nanovectors holds great promise and heralds a new era of dendrimer-mediated delivery of RNA therapeutics in biomedical applications.

Keywords： gene therapy     RNAi therapeutics     dendrimer     nanovectors     gene silencing    

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The antibiotic resistome: gene flow in environments, animals and human beings

Yongfei Hu, George F. Gao, Baoli Zhu

Frontiers of Medicine 2017, Volume 11, Issue 2,   Pages 161-168 doi: 10.1007/s11684-017-0531-x

Abstract: We propose that the resistance gene flow in nature is “from the natural environments” andand animals, as intermediate recipients and disseminators, contribute greatly to such a resistance gene

Keywords： antibiotic resistance     resistome     microbiome     gene flow    

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Reflections on the system of evaluation of gene-edited livestock

Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 2,   Pages 211-217 doi: 10.15302/J-FASE-2019303

Abstract: functions regarding economic traits, and new materials for livestock breeding have been obtained through geneEvaluation is the most important component of the regulation of gene-edited livestock and is a crucialguarantee of public safety before the marketing of gene-edited animal products.Here, the system of evaluation of gene-edited livestock is discussed in terms of public safety and production

Keywords： evaluation     gene editing     livestock     performance     safety    

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Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 141-147 doi: 10.1007/s11684-009-0041-6

Abstract: To explore the possibility of Rab9-related gene therapy for neurodegenerative diseases, we packed LentivirusWestern blotting revealed that the Rab9 gene expression in BALB/c mice brain was up-regulated significantlyLentivirus encoding Rab9 can increase the expression of Rab9 gene effectively, which might offer a novel

Keywords： Rab9     lentivirus     gene therapy     gene transfer    

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Gene delivery into isolated

Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 521-528 doi: 10.1007/s11705-017-1612-8

Abstract: The application of gene delivery materials has been mainly focused on mammalian cells while rarely extendedThis study therefore widens the utilities of helical polypeptide as a unique category of gene deliverymaterials, and may find their promising applications toward plant gene delivery.

Keywords： α-helical polypeptide     plant gene delivery     protoplast     intact leaves     transfection    

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Profiling influences of gene overexpression on heterologous resveratrol production in

Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 117-125 doi: 10.1007/s11705-016-1601-3

Abstract: We overexpressed the main gene targets in the upstream pathways to investigate their influences on theSingle-gene overexpression and DNA assembly-directed multigene overexpression affect the production ofFinally, the collaboration of selected gene targets leads to an optimal resveratrol production of 66.14The newly discovered gene targets expressing phosphoribosylanthranilate isomerase, expressing 3-deoxy-D-arabino-heptulosonate

Keywords： resveratrol     aromatic amino acid     DNA assembly     metabolic engineering     gene overexpression    

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Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity

Chuanfeng Wu, Cynthia E. Dunbar

Frontiers of Medicine 2011, Volume 5, Issue 4,   Pages 356-371 doi: 10.1007/s11684-011-0159-1

Abstract: Virus-based vectors are widely used in hematopoietic stem cell (HSC) gene therapy, and have the abilityTo date, HSC gene therapy has been successfully employed in the clinic for improving clinical outcomesHowever, adverse events were observed during some of these HSC gene therapy clinical trials, linked tomechanisms of insertional mutagenesis in hematopoietic stem and progenitor cells due to integrating gene, and introduce newly-developed approaches for minimizing genotoxicity as a way to further move HSC gene

Keywords： gene therapy     hematopoietic stem cells     insertional mutagenesis     genotoxicity     induced pluripotent stem cell    

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Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Frontiers of Medicine 2015, Volume 9, Issue 1,   Pages 90-99 doi: 10.1007/s11684-015-0390-2

Abstract:

Gene therapy provides a potential cure for hemophilia B, and significant progress has been achievedin liver-directed gene transfer mediated by adeno-associated viral vectors.vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B geneand adjusting the GC content, inserting a Kozak sequence (GCCACC), and introducing a gain-of-function mutationIn addition, the regulatory elements that control the FIX gene expression in these cassettes

Keywords： factor IX     hemophilia B     liver-specific regulatory elements     hydrodynamic gene transfer    

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Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome

Lu Wang

Frontiers of Medicine 2013, Volume 7, Issue 3,   Pages 280-289 doi: 10.1007/s11684-013-0265-3

Abstract:

Many gene fusions have been recognized as important diagnostic and/or prognostic markers in humanIn recent years, novel gene fusions have been identified in cases without prior knowledge of the geneticThis review focused on advanced genome-wide screening approaches in fusion gene identification, suchas microarray-based approaches, next-generation sequencing, and NanoString nCounter gene expression systemThe fundamental rationale and strategy for fusion gene identification using each biotech platform are

Keywords： gene fusion     cancer     microarray     next-generation sequencing     NanoString nCounter system    

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Title Author Date Type Operation

Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation

Journal Article

Mutation profiling of 16 candidate genes in

Yang Zhang, Fang Wang, Xue Chen, Wenjing Liu, Jiancheng Fang, Mingyu Wang, Wen Teng, Panxiang Cao, Hongxing Liu

Journal Article

662 A/G gene variation in human tumor necrosis factor receptor superfamily, member 9 (TNFRSF9)

QU Yanchun, YANG Ze, SUN Liang, JI Linong

Journal Article

Analysis of the genomic landscape of primary central nervous system lymphoma using whole-genome sequencing in Chinese patients

Journal Article

Novel mutation c.1210-3C>G in with a poly-T tract of 5T affects mRNA splicing in a Chinese patient

Journal Article

Study on Family Collection, Gene Mapping, Gene Identification and Gene Function of Human Genetic Diseases

Xia Jiahui

Journal Article

Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing

Yu Cao, Xiaoxuan Liu, Ling Peng

Journal Article

The antibiotic resistome: gene flow in environments, animals and human beings

Yongfei Hu, George F. Gao, Baoli Zhu

Journal Article

Reflections on the system of evaluation of gene-edited livestock

Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI

Journal Article

Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain

Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI

Journal Article

Gene delivery into isolated

Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng

Journal Article

Profiling influences of gene overexpression on heterologous resveratrol production in

Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan

Journal Article

Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity

Chuanfeng Wu, Cynthia E. Dunbar

Journal Article

Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B

Ru Zhang,Qiang Wang,Lin Zhang,Saijuan Chen

Journal Article

Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome

Lu Wang

Journal Article